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Engineering zinc-finger transcription factors (Cardiff University) We are currently working on a BBSRC funded project (BB/E013503/1) to engineer the DNA binding domain of zinc-finger transcription fingers to switch its binding on/off in response to a small molecule effector. The new proteins are being designed with a small molecule binding site in one of the fingers, and the residues lining this binding site will be optimized using a phage display procedure. This approach allows us to choose the small molecule effector at will to obtain desirable in vivo properties (bioavailability, low toxicity). |
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Above - Switchable zinc finger protein binds to DNA in response to a small molecule effector. |
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[James Redman] [Research] [Porphyrins] [Peptides] [Modelling] [Proteins] [Laboratory] [Publications] [Animations] [Wales] [Links] [Contact] |
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Copyright ©2010 James E. Redman. All rights reserved. |